| 产品编号 | bs-105594P |
| 英文名称 | Recombinant Mouse CD74 Protein, C-His |
| 中文名称 | |
| 别 名 | H-2 class II histocompatibility antigen gamma chain; Ia antigen-associated invariant chain; Ii; Ii chain; MHC class II-associated invariant chain; CD74; Class-II-associated invariant chain peptide; CLIP; Cd74 |
| 克 隆 号 | |
| 理论分子量 | 28.34 kDa |
| 检测分子量 | |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of, or in association with, MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] SWISS: P04441 Gene ID: 16149 CD74表达于所有B细胞和某些T细胞亚类（MHC II阳性），具有几种异构体形式（33，35和41kDa），是HLA-DR的恒定链。该抗体主要用于标记生发中心及外套B细胞（80%）和R-S细胞（70%）以及少数T细胞（20%），常与其它B细胞标记如CD20或CD45RA联合检测，用于B细胞淋巴瘤的辅助诊断。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
