| 产品编号 |
bs-0362P-Cy5 |
| 英文名称 |
Recombinant protein A, Cy5 conjugated
|
| 中文名称 |
Cy5标记的重组蛋白A/重组金黄色葡萄球菌蛋白A |
| 别 名 |
Immunoglobulin G-binding protein A; SPA; Recombinant protein A; Staphylococcal protein A; IgG-binding protein A; SPA_STAA8; ProteinA recA蛋白; 重组蛋白-A; |
| 克 隆 号 |
|
| 理论分子量 |
34 kDa |
| 检测分子量 |
45-47 |
| 性 状 |
Liquid |
| 浓 度 |
1mg/ml |
| 纯 度 |
>95% as determined by SDS-PAGE |
| 缓 冲 液 |
10 mM TBS (pH=7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 |
Stored at -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.
产品说明 蛋白A是从非致病性Bacillus中获取的通过基因工程生产的重组蛋白，与多数哺乳动物的IgG Fc段结合（包括：人、山羊、绵羊、兔、豚鼠、马、猪、猴、小鼠等）。不与狗IgG结合、不结合人IgM、IgD、和IgA。重组蛋白A（Recomb Protein A）已经除去了与白蛋白及细胞表面结合位点，减少了交叉反应和非特异性结合。 因此，它比天然蛋白G和蛋白A有更大的亲和力。可以代替二抗，广泛应用于免疫化学等领域。在亲和力、稳定性等方面好。
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