| 产品编号 | bs-42594P |
| 英文名称 | Recombinant human PIK3CA protein, N-His |
| 中文名称 | 重组人磷脂酰肌醇激酶催化亚单位A蛋白 |
| 别 名 | PK3CA_HUMAN; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PIK3CA; EC:2.7.1.137; EC:2.7.1.153; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha; Phosphoinositide 3-kinase alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA; EC:2.7.11.1; HMH; MCM; CCM4; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; p110-alpha; |
| 克 隆 号 | |
| 理论分子量 | 44.9 kDa |
| 性 状 | Liquid |
| 纯 度 | >90% as determined by SDS-PAGE |
| 缓 冲 液 | 20MMTris-HCl (pH=9.0) with 8M Urea, 150mM NaCL,5mM DTT |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdInsP2. Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach. SWISS: P42336 Gene ID: 5290 磷脂酰肌醇激酶（P110α亚单位） |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
