| 产品编号 | bs-48106P |
| 英文名称 | Recombinant human FGF-2/bFGF protein (Active, E.coli) |
| 中文名称 | 重组人碱性成纤维细胞生长因子 |
| 别 名 | Basic fibroblast growth factor; FGF basic; FGF-basic; BFGF; FGF-2; FGF B; FGF2 basic; FGFB; Fibroblast growth factor 2 (basic); HBGF 2; HBGF-2; HBGF2; HBGH 2; HBGH2; Heparin binding growth factor 2 precursor; Prostatropin; FGF2_HUMAN; |
| 克 隆 号 | |
| 理论分子量 | 16.5 kDa |
| 性 状 | Lyophilized |
| 纯 度 | ≥ 95% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a 0.2μm filtered solution containing PB, 5% Trehalose and 0.01% Tween 80, pH7.4 |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 36 months at -20℃ to -80℃ in lyophilized state. 6 months at -20℃ to -80℃ under sterile conditions after reconstitution. 7-10 days at 2℃ to 21℃ under sterile conditions after reconstitutio |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]. SWISS: P09038 Gene ID: 2247 生长因子和激素（ Growth Factor and Hormones） (b-FGF)主要分布于垂体、脑和神经组织及视网膜、肾上腺、胎盘等，尤以垂体含量最高。 FGF2(basic fibroblast growth factor)碱性成纤维细胞生长因子是由内皮细胞及平滑肌细胞合成并对合成细胞有促分裂效应的多肽分子,具有强烈的血管生成作用，参与血管生成、组织修复、神经系统的发育等 。FGF2在体外，能刺激细胞增殖，细胞迁移，诱导纤溶酶原激活物及胶原酶活性。是能与肝素结合的促分裂剂。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
