| 产品编号 | bs-100868P-Bio |
| 英文名称 | Recombinant Human CD13/ANPEP Protein, N-His, Biotin conjugated |
| 中文名称 | |
| 别 名 | Aminopeptidase M; APN; AP-M; gp150; hAPN; AP-N; PEPN; Microsomal aminopeptidase; CD13; Aminopeptidase N; Alanyl aminopeptidase; Myeloid plasma membrane glycoprotein CD13; ANPEP |
| 克 隆 号 | |
| 理论分子量 | 21.01 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS(pH 7.4) with 5% Trehalose. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma. [provided by RefSeq, Apr 2020] SWISS: P15144 Gene ID: 290 氨肽酶N(又称CD13)是氨肽酶系列的一种，氨肽酶是从蛋白质多肽链氨基端催化降解氨基酸残基的水解蛋白酶。是多种冠状病毒的受体，目前在肿瘤侵袭、转移、免疫调节和病毒感染等多方面受人们的关注.它在肿瘤细胞表面高水平表达,对肿瘤细胞外基底膜起到降解作用引发肿瘤的侵袭和转移。主要分布于小肠和肾脏，巨噬细胞、粒细胞和中枢神经系统的突触膜也表达CD13. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
