| 产品编号 | bs-42390P |
| 英文名称 | Recombinant human PRKACA protein, Tag free |
| 中文名称 | 重组人蛋白激酶A催化亚基蛋白 |
| 别 名 | KAPCA_HUMAN; cAMP-dependent protein kinase catalytic subunit alpha; EC:2.7.11.11; PKA C-alpha; PKACA; |
| 理论分子量 | 40.7kDa |
| 性 状 | Liquid |
| 浓 度 | >1mg/ml |
| 纯 度 | >90% as determined by SDS-PAGE |
| 缓 冲 液 | 10mM PB (pH=7.4) with 0.15M NaCL, 0.1mM EGTA, 0.1mM EDTA, 25% Glycerol |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKA beta (catalytic subunit) is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. SWISS: P22694 Gene ID: 5567 激酶和磷酸酶（Kinases and Phosphatases） 蛋白激酶 A (protein kinase A，PKA) 又称依赖于cAMP的蛋白激酶A (cyclic-AMP dependent protein kinase A)，是一种结构最简单、生化特性最清楚的蛋白激酶。 蛋白激酶A属于多功能丝氨酸和苏氨酸激酶,可使蛋白磷酸化，也可自身磷酸化。在调节细胞存活中起重要作用。 |
| 产品图片 |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
