| 产品编号 | bsm-63770R |
| 英文名称 | p14ARF Recombinant Rabbit mAb |
| 中文名称 | |
| 别 名 | cyclin dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); CDKN2A/ARF Intron 2 lncRNA; multiple tumour suppressor 1; cyclin-dependent kinase 4 inhibitor A; p19 alternate open reading frame; p14 alternate open reading frame; inhibitor of cdk4 A; Cyclin-dependent kinase inhibitor 2A isoform 4; CDKN2A; CDKN2; MLM; Cyclin-dependent kinase inhibitor 2A; isoform 4; p14ARF; p19ARF |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 1C9 |
| 交叉反应 | Human |
| 产品应用 | WB=1:2000-10000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000,ELISA=1:5000-20000,IP=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 |
CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by CDKN2A, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. CDKN2A is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. SWISS: Q8N726 Gene ID: 8350 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
