| 产品编号 | bsm-63491R |
| 英文名称 | SPARC Recombinant Rabbit mAb |
| 中文名称 | |
| 别 名 | BM-40; OI17; ON; ONT; SPRC_HUMAN; SPARC; Basement-membrane protein 40 (BM-40); Osteonectin (ON); Secreted protein acidic and rich in cysteine; secreted protein acidic and cysteine rich; osteonectin; cysteine-rich protein; Basement-membrane protein 40 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 1D1 |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:1000-5000,IHC-P=1:200-500,IHC-F=1:200-500,IF=1:200-1000,ELISA=1:5000-20000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015], SWISS: P09486 Gene ID: 6678 SPARC是从多方面调节细胞功能的细胞外基质蛋白，SPARC蛋白在人组织中广泛分布与组织重建和肿瘤有关。SPARC在多种肿瘤中呈高表达. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
