| 产品编号 | bsm-63517R |
| 英文名称 | RPA2 Recombinant Rabbit mAb |
| 中文名称 | |
| 别 名 | REPA2; RP-A p32; RP-A p34; RPA32; RPA34; Rf-A2; RFA2_HUMAN; RPA2; Replication factor A protein 2 (RF-A protein 2); Replication protein A 34 kDa subunit (RP-A p34); RFA2_MOUSE; replication protein A2; replication protein A2 (32kD); replication protein A2, 32kDa; Replication protein A 32 kDa subunit; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 11B7 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:2000-10000,IHC-P=1:1000-4000,IHC-F=1:1000-4000,IF=1:200-1000,ELISA=1:5000-20000,IP=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 |
function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN., SWISS: P15927 Gene ID: 6118 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
