| 产品编号 | bsm-63587R |
| 英文名称 | FEN1 Recombinant Rabbit mAb |
| 中文名称 | |
| 别 名 | FEN-1; FEN1_HUMAN; FEN1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1 (MF1 | hFEN-1); 3.1.-.-; RAD2; FEN1_MOUSE; MF1; maturation factor-1; Flap endonuclease 1; Maturation factor 1; hFEN-1 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 4H7 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:2000-10000,IHC-P=1:500-2000,IHC-F=1:500-2000,IF=1:200-1000,ELISA=1:5000-20000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions SWISS: P39748 Gene ID: 2237 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
