| 产品编号 | bsm-63619R |
| 英文名称 | MeCP2 Recombinant Rabbit mAb |
| 中文名称 | |
| 别 名 | AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT; 1500041B07Rik; D630021H01Rik; Mbd5; WBP10; MECP2_e1; MECP2_HUMAN; MECP2; MeCp-2 protein; MECP2_MOUSE; MECP2_RAT; methyl-CpG binding protein 2; mental retardation, X-linked 16; mental retardation, X-linked 79; Rett syndrome; methyl CpG binding protein 2 (Rett syndrome); methyl CpG binding protein 2; Methyl-CpG-binding protein 2 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 4D5 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:1000-5000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000,ELISA=1:5000-20000,IP=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 |
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isofor SWISS: P51608 Gene ID: 4204 MECP2蛋白是一种丰富的染色质结合蛋白,属于DNA结合蛋白大家族中一员,是一种转录抑制因子,能选择性地与甲基化的CpG(甲基胞嘧啶)结合,特别是与含单个甲基化的CpG结合。MECP2的突变可以导致持续性神经系统退化（neurodegenerative disorder）Rett综合症。这个x-染色体相关综合症（X-linked syndrome）主要在女性身上体现， 他突变也可以导致男性的智力障碍，Rett综合征是一种严重影响儿童精神运动发育的神经遗传病。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
