| 产品编号 | bs-0837R-APC |
| 英文名称1 | Rabbit Anti-Glutathione Reductase/APC Conjugated antibody |
| 中文名称 | APC标记的谷胱甘肽还原酶抗体 |
| 别 名 | glutathione reductase; GLUR; Glutathione reductase mitochondrial; GR; Gr1; GRase; GRD 1; GRD1; GSR; MGC78522; GSHR_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 免疫学 激酶和磷酸酶 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 57kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glutathione Reductase |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Glutathione reductase (GR) is a member of pyridine nucleotide- disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. Function: Maintains high levels of reduced glutathione in the cytosol. Subunit: Homodimer; disulfide-linked. Subcellular Location: Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. Database links: Entrez Gene: 2936 Human Entrez Gene: 14782 Mouse Omim: 138300 Human SwissProt: P00390 Human SwissProt: P47791 Mouse Unigene: 271510 Human Unigene: 283573 Mouse Unigene: 19721 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 胱甘肽还原酶为一种黄素酶，可催化氧化型谷胱甘肽转变成还原型谷胱甘肽(GSH)，从而提高细胞液中GSH的含量。 GSH具有抵抗自由基氧化对机体损伤的作用，GSH的重要生理功能在于能还原体内生成的过氧化氢(H2O2)。GSH的储量与创伤、肿瘤等的预后，以及人体衰老有关。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
