| 产品编号 | bs-9901R-Bio |
| 英文名称 | Anti-PAFAHG/Biotin |
| 中文名称 | 生物素标记的血小板衍化生长因子γ抗体 |
| 别 名 | PA1B3_HUMAN; PAF acetylhydrolase 29 kDa subunit; PAF AH 29 kDa subunit; PAF AH subunit gamma; PAF-AH 29 kDa subunit; PAF-AH subunit gamma; PAFAH subunit gamma; Pafah1b3; PAFAHG; Platelet activating factor acetylhydrolase IB subunit gamma; Platelet activating factor acetylhydrolase isoform Ib gamma subunit (29kD); Platelet activating factor acetylhydrolase isoform Ib gamma subunit 29kDa; Platelet activating factor acetylhydrolase isoform Ib gamma subunit; Platelet-activating factor acetylhydrolase IB subunit gamma. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 心血管 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 26kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PAFAH1B3/PAFAHG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: The platelet activating factor (PAF) acetylhydrolases catalyze hydrolysis of the sn-2 ester bond of PAF and related pro-inflammatory phospholipids and thus attenuate their bioactivity. The family of PAF acetylhydrolases includes one secreted plasma isozyme and two intracellular isozymes. The intracellular isozymes are distinguished by differences in their primary sequence, tissue localization, subunit composition and substrate preferences. The most thoroughly characterized intracellular isoform, PAFAH1B, is a heterotrimeric protein expressed in brain tissue and plays and important role in brain development and function. PAFAH1B is comprised of a regulatory subunit (LIS1) and two homologous (63% identity) catalytic subunits (PAFAH1B2 and PAFAH1B3), which harbor all the activity of the enzyme. The PAFAH1B2 and PAFAH1B3 subunits readily associate with very high affinity to form heterodimers, and this dimerization is essential for both stability and catalytic activity. PAFAH1B3 is also commonly known as PAFAH1B 29kDa subunit, PAFAH1B subunit or PAFAH1B subunit α1. Function: Inactivates paf by removing the acetyl group at the sn-2position. This is a catalytic subunit. Plays an important roleduring the development of brain. Subunit: Cytosolic PAF-AH IB is formed of three subunits of 45 kDa(alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activityof the enzyme resides in the beta and gamma subunits, whereas thealpha subunit has regulatory activity. Trimer formation is notessential for the catalytic activity. Subcellular Location: Cytoplasm. Tissue Specificity: In the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain. Similarity: Belongs to the 'GDSL' lipolytic enzyme family.Platelet-activating factor acetylhydrolase IB beta/gamma subunitssubfamily. Database links: Entrez Gene: 5050 Human Entrez Gene: 18476 Mouse Omim: 603074 Human SwissProt: Q15102 Human SwissProt: Q61205 Mouse Unigene: 466831 Human Unigene: 597 Mouse Unigene: 17971 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
