| 产品编号 | bs-0646R-FITC |
| 英文名称 | Anti-CD34/FITC |
| 中文名称 | FITC标记的CD34抗体 |
| 别 名 | CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN. |
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Journal
PMID
IF
Application
[IF=6.543] Liang Guibin. et al. lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients. Oxid Med Cell Longev. 2022;2022:9369035 FC ; Human.
[IF=5.352] Tianli Yang. et al. Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders:. J Tissue Eng. 2021;(): FC ; Rat.
[IF=4.067] Kong L et al. Silencing MicroRNA-137-3p, which Targets RUNX2 and CXCL12 Prevents Steroid-induced Osteonecrosis of the Femoral Head by Facilitating Osteogenesis and Angiogenesis. Int J Biol Sci. 2020 Jan 14;16(4):655-670. FCM ; Rat.
[IF=3.923] Li H et al. siRNA‐mediated silencing of PAI‐1 gene acts as a promoter over the recanalization of endothelial progenitor cells in rats with venous thrombosis. J Cell Physiol. 2019 Apr 14. ICF ; Rat.
[IF=3.553] Chen L et al. Role of complement 3 in renin generation during the differentiation of mesenchymal stem cells to smooth muscle cells. Am J Physiol Cell Physiol. 2020 May 1;318(5):C981-C990. FACS ; mouse.
[IF=3.21] Yu, Jie, et al. "Activation of liver X receptor enhances the proliferation and migration of endothelial progenitor cells and promotes vascular repair through PI3K/Akt/eNOS signaling pathway activation." Vascular Pharmacology (2014). FCM ; Rat.
[IF=2.548] Dworacka M et al. Simvastatin attenuates the aberrant expression of angiogenic factors induced by glucose variability.Diabetes Res Clin Pract. 2018 Sep;143:245-253. FCM ; Rat.
[IF=2.447] Xuejun Dai. et al. Comparison of the differentiation abilities of bone marrow‑derived mesenchymal stem cells and adipose‑derived mesenchymal stem cells toward nucleus pulposus‑like cells in three‑dimensional culture. Exp Ther Med. 2021 Sep;22(3):1-9 FC ; Rat.
[IF=2.199] Nau C et al. Influence of the induced membrane filled with syngeneic bone and regenerative cells on bone healing in a critical size defect model of the rat's femur.Injury. 2018 Oct;49(10):1721-1731. FCM ; Rat.
[IF=2.1] Hongxia Wei. et al. MRI tracing of ultrasmall superparamagnetic iron oxide nanoparticle‑labeled endothelial progenitor cells for repairing atherosclerotic vessels in rabbits. Mol Med Rep. 2020 Oct;22(4):3327-3337 FC ; Rabbit.
[IF=0.94] Bi, Lingyun, et al. "Effect of bone marrow stem cell mobilisation on the expression levels of cellular growth factors in a rat model of acute tubular necrosis." Experimental and Therapeutic Medicine.10(2):618-624 FCM ; Rat.
[IF=0] Shereen Morsy. et al. Can mobilization of bone marrow stem cells be an alternative regenerative therapy to stem cell injection in a rat model of chronic kidney disease?. PHYSIOL REP. 2022 Sep;10(17):e15448 FC ; Rat.
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| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 心血管 免疫学 发育生物学 神经生物学 干细胞 细胞表面分子 糖蛋白 细胞类型标志物 血管内皮细胞 内皮细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Rabbit, ) |
| 产品应用 | Flow-Cyt=2ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 39kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD34 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. Function: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Post-translational modifications: Highly glycosylated. Phosphorylated on serine residues by PKC. Similarity: Belongs to the CD34 family. Database links: Entrez Gene: 947 Human Omim: 142230 Human SwissProt: P28906 Human Unigene: 374990 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 造血干细胞标志物 内皮标志物 肿瘤生物标志物 细胞表面的唾液粘蛋白。 间充质干细胞（mesenchymal stem cells,MSC）也是干细胞家族的重要成员，来源于发育早期的中胚层和外胚层。MSC最初在骨髓中发现，因其具有多向分化潜能、造血支持和促进干细胞植入、免疫调控和自我复制等特点。如间充质干细胞在体内或体外特定的诱导条件下，可分化为血管内皮、脂肪、骨、软骨、肌肉、肌腱、韧带、神经、肝、心肌、等多种组织细胞。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-CD34 Antibody, conjugated (bs-0646R-FITC) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-CD34 antibody (bs-0646R-PE)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
