| 产品编号 | bs-3461R-Gold |
| 英文名称 | Anti-Phospho-PPIG (Ser376)/Gold |
| 中文名称 | 胶体金标记的磷酸化亲环蛋白（亲环素）PPIG抗体 |
| 别 名 | CARS cyclophilin; CARS Cyp; CASP 10; CASP10; Clk associating RS cyclophilin; Cyclophilin G; CyclophilinG; CYP; MGC133241; Peptidyl prolyl cis trans isomerase G; Peptidyl prolyl isomerase G; Peptidylprolyl isomerase G (cyclophilin G); Peptidylprolyl isomerase G; PPIase G; Rotamase G; RotamaseG; PPIG_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul（10nm 15nm 35nm） |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 89kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PPIG around the phosphorylation site of Ser376 [VS(p-S)GE] |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 产品介绍 |
background: PPIG belongs to the the peptidyl-prolyl cis/trans isomerase protein family (PPIases). PPIases accelerate the folding of proteins. PPIG catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is distributed in nuclear speckles, a nuclear compartment rich in splicing factors, and colocalizes with the splicing factors SC35 and pinin, suggesting a role in the regulation of pre-mRNA splicing. Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing. Subunit: Interacts with CLK1, PNN and with the phosphorylated C-terminal domain of RNA polymerase II. Subcellular Location: Nucleus matrix. Nucleus speckle. Note=Colocalizes with RNA splicing factors at nuclear speckles. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 PPIase cyclophilin-type domain. Database links: Entrez Gene: 9360 Human Entrez Gene: 228005 Mouse Omim: 606093 Human SwissProt: Q13427 Human SwissProt: A2AR02 Mouse Unigene: 727580 Human Unigene: 11815 Mouse Unigene: 474951 Mouse Unigene: 10898 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 肽基脯氨酰基顺反异构酶，广泛存在于各种组织和器官。根据对免疫抑制剂的敏感性，分属三个独立的家族：亲环蛋白PPIG、FK506结合蛋白和细蛋白。能够加速自身折叠和体内新生肽链折叠及变性蛋白质的体外折叠，主要分布于内质网。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
