| 产品编号 | bs-0348R-BF350 |
| 英文名称 | Rabbit Anti-GNB1/BF350 Conjugated antibody |
| 中文名称 | BF350标记的G蛋白/鸟苷酸结合蛋白抗体 |
| 别 名 | G protein beta subunit GI/GS/GT; guanine nucleotide binding protein (G protein), beta polypeptide 1; guanine nucleotide-binding protein G; GBB1_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 37kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G protein beta subunit GI |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: G protein beta subunit GI/GS/GT (guanine nucleotide-binding protein G). WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment. Function: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Subunit: G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus). Post-translational modifications: Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP. Similarity: Belongs to the WD repeat G protein beta family. Contains 7 WD repeats. Database links: Entrez Gene: 2782 Human Entrez Gene: 14688 Mouse Omim: 139380 Human SwissProt: P62873 Human SwissProt: P62874 Mouse Unigene: 430425 Human Unigene: 2344 Mouse Unigene: 126047 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. G蛋白又称：鸟苷酸结合蛋白，存在于全身各个组织和细胞中，G蛋白在信息传递中起到很重要的作用,参与细胞内外信息的相互传导。 激素受体与腺苷酸环化酶是细胞膜上两类分开的蛋白质。激素受体结合的部分在细胞膜的外表面，而腺苷环化酶在膜的胞浆面，在两者之间存在一种起耦联作用的调节蛋白——鸟苷酸结合蛋白（guanine nucleotide-binding regulatory protein）细胞受到各种刺激信息，包括化学性的激素和神经递质及非化学性的声音、图像等。刺激信息通过G蛋白的激活作用，在胞浆中生成数目众多的第二信使分子，产生十万级放大效应，引起细胞兴奋，让后促动器官完成各自功能。 1977年，美国科学家阿尔弗雷德-戈德曼-吉尔曼(Alfred Goodman Gilman)发现了G蛋白及其在细胞兴奋传导方面的重要作用。1994年，吉尔曼因“G蛋白”方面的研究成果而荣获诺贝尔生理及医学奖。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
