| 产品编号 | bs-1030R-AF647 |
| 英文名称 | Anti-HMGA1/AF647 |
| 中文名称 | AF647标记的高迁移率族蛋白A1抗体 |
| 别 名 | HMG-I; HMG-Y; High mobility group AT-hook 1; HMG-I/HMG-Y; HMG-R; HMGA 1a; HMGA 1b; HMGA1; HMGI; HMGI/Y; HMGIY; HMGY; MGC12816; MGC4242; MGC4854; HMGA1_HUMAN; High mobility group protein HMG-I/HMG-Y; HMG-I(Y); AltName: Full=High mobility group AT-hook protein 1; High mobility group protein A1; High mobility group protein R. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 染色质和核信号 微生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, Cow, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 12kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HMGA1 (51-107aa) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: HMGA1 (HMGI/Y; high mobility group AT-hook 1) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex. Function: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. Subunit: Interacts with HIPK2 (By similarity). Interacts with HIV-1 pre-integration complex. Subcellular Location: Nucleus. Chromosome. Post-translational modifications: Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity. HMG-Y is not methylated. Methylation at Arg-58 is mutually exclusive with methylation at Arg-60. DISEASE: Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Similarity: Belongs to the HMGA family. Contains 3 A.T hook DNA-binding domains. Database links: Entrez Gene: 3159 Human Entrez Gene: 15361 Mouse Omim: 600701 Human SwissProt: P17096 Human SwissProt: P17095 Mouse Unigene: 518805 Human Unigene: 4438 Mouse Unigene: 228608 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
