| 产品编号 | bs-4082R-AF350 |
| 英文名称 | Anti-phospho-LATS2 (Ser83)/AF350 |
| 中文名称 | AF350标记的磷酸化肿瘤抑制基因LATS2抗体 |
| 别 名 | Kinase phosphorylated during mitosis; KPM; Large tumor suppressor homolog 2; Large tumor suppressor homolog 2 Drosophila; LATS large tumor suppressor Drosophila homolog 2; LATS large tumor suppressor homolog 2; Serine/threonine kinase kpm; Serine/threonine protein kinase kpm; Warts like kinase; FLJ13161; Kinase phosphorylated during mitosis protein; Large tumor suppressor kinase 2; Lats2; LATS2_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 信号转导 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, ) |
| 产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 120-150kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human LATS2 around the phosphorylation site of Ser83 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: LATS2 is a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Subunit: Interacts with and is phosphorylated by AURKA. Binds to AR. Interacts with AJUBA during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. Interacts (via PPxY motif) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Tissue Specificity: Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 UBA domain. Database links: Entrez Gene: 26524 Human Entrez Gene: 50523 Mouse Omim: 604861 Human SwissProt: Q9NRM7 Human SwissProt: Q7TSJ6 Mouse Unigene: 78960 Human Unigene: 347899 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
