产品编号 | bs-0628R-AF594 |
英文名称 | Anti-ZNF300/AF594 |
中文名称 | AF594标记的锌指蛋白300抗体 |
别 名 | Kruppel like zinc finger protein; Zinc finger protein 300; ZN300_HUMAN. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 染色质和核信号 信号转导 转录调节因子 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse, (predicted: Human, ) |
产品应用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 68kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF300 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: ZNF300 contains C2H2 (or Kruppel)-type zinc finger motifs that tetrahedrally coordinate zinc ions and form a protein structure that can bind DNA and regulate transcription (Gou et al.,2004 [PubMed 14746915]). ZNF300 belongs to the krueppel C2H2 type zinc finger protein family. It contains twelve C2H2 type zinc fingers and one KRAB domain. ZNF300 has a transcriptional repressor activity. Expressed mostly in heart, skeletal muscle and brain. Isoforms 1 and 2 are highly expressed in testis. There are three named isoforms. Function: Has a transcriptional repressor activity. Subcellular Location: Nucleus. Tissue Specificity: Expressed mostly in heart, skeletal muscle and brain. Isoform 1 and isoform 2 are highly expressed in testis. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 12 C2H2-type zinc fingers. Contains 1 KRAB domain. Database links: Entrez Gene: 91975 Human Omim: 612429 Human SwissProt: Q96RE9 Human Unigene: 134885 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ZNF300白血病相关性研究。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |