| 产品编号 | bs-11968R-PE-Cy5 |
| 英文名称 | Rabbit Anti-Semaphorin 3D/PE-Cy5 Conjugated antibody |
| 中文名称 | PE-Cy5标记的跨膜蛋白SEMA3D抗体 |
| 别 名 | Coll 2; Coll2; Collapsin 2; SEMA3D; Semaphorin3D; SEM3D_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 发育生物学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Horse, Rabbit, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 85kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Semaphorin 3D |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. SEMA3D (semaphorin-3D), also known as coll-2 or Sema-Z2, is a 777 amino acid secreted protein that belongs to the semaphorin family and participates in axon-axon interactions and neural crest activity. SEMA3D binds neuropilin and is able to induce the collapse and paralysis of neuronal growth cones, as well as act to repel specific neuronal populations. SEMA3D contains one immunoglobulin-like (Ig-like) domain, one PSI domain and one semaphorin domain. Function: One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. The class 3 semaphorins belong to the secreted group. Via interaction with its neuropilin receptor, semaphorin 3D plays a specific role in the induction of paralysis and collapse of neuronal growth cones. Semaphorin 3D could potentially act as repulsive cues toward specific neuronal populations. Subcellular Location: Secreted Similarity: Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 PSI domain. Contains 1 Sema domain. Database links: Entrez Gene: 223117 Human Entrez Gene: 108151 Mouse Omim: 609907 Human SwissProt: O95025 Human SwissProt: Q8BH34 Mouse Unigene: 201340 Human Unigene: 89313 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
