| 产品编号 | bs-12487R-Cy5.5 |
| 英文名称 | Rabbit Anti-APG8A/Cy5.5 Conjugated antibody |
| 中文名称 | Cy5.5标记的自噬相关蛋白8A抗体 |
| 别 名 | APG8A (autophagy 8A); AT4G21980; ATG8; ATG8A; Autophagy 8A; Autophagy related protein 8a; Autophagy related ubiquitin like modifier ATG8a; F1N20.80; F1N20_80; ATG8A_ARATH. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 转运蛋白 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 13kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis thaliana APG8A |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈. |
| 产品介绍 |
background: APG8A is involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. It may mediate the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton. Function: Involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. May mediate the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton. Subunit: Interacts with ATG4b. Subcellular Location: Cytoplasmic vesicle ?cvt vesicle membrane; Lipid-anchor. Cytoplasmic vesicle ?autophagosome membrane; Lipid-anchor. Vacuole membrane; Lipid-anchor. Note= Membrane-associated through a lipid anchor. Post-translational modifications: The C-terminal 5 residues are removed by ATG4 to expose Gly-117 at the C-terminus. This Gly-117 forms then a thioester bond with the 'Cys-558' of ATG7 (E1-like activating enzyme) before being transferred to the 'Cys-258' of ATG3 (the specific E2 conjugating enzyme), in order to be finally amidated with phosphatidylethanolamine. This lipid modification anchors ATG8 to autophagosomes (By similarity). Similarity: Belongs to the MAP1 LC3 family. Database links: UniProtKB/Swiss-Prot: Q8LEM4.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
