| 产品编号 | bs-14528R-AF555 |
| 英文名称1 | Rabbit Anti-EHD4/AF555 Conjugated antibody |
| 中文名称 | AF555标记的EHD4蛋白抗体 |
| 别 名 | EH domain containing 4; EH domain containing protein 4; EH domain-containing protein 4; EH domaincontaining protein 4; EH-domain containing 4; EHdomain containing 4; EHdomain containing4; Hepatocellular carcinoma associated protein 10/11; hepatocellular carcinoma-associated protein 10/11; hepatocellular carcinoma-associated protein HCA11; HGNC:3245; EHD4_HUMAN; Ortholog of rat pincher; PAST homolog 4; PAST4. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 心血管 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Horse, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 61kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EHD4 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: EHD4 (Eps15 homology domain-containing protein 4), also known as PAST4 or Pincher, belongs to a subfamily of the EHD protein family that includes the closely related proteins EHD1, EHD2 and EHD3. EHD4 is predominantly expressed in pancreas and heart localizing to vesicular and tubular structures in the cell. It contains an EH domain as well as a calcium binding EF hand. EHD4 is believed to function in transport from the early endosome to the endocytic recycling compartment. In addition, EHD4 is capable of binding lipids via its EH domain. Loss of EHD4 can lead to retention of transferrin in peripheral compartments suggesting that EHD4 regulates the transport of transferrin out of the early endosome. Function: EHD4 is involved in the control of trafficking at the early endosome and regulates exit of cargo toward both the recycling compartment and the late endocytic pathway. Subcellular Location: Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Similarity: Contains 1 EF-hand domain. Contains 1 EH domain. Database links: Entrez Gene: 30844 Human Entrez Gene: 98878 Mouse Omim: 605892 Human SwissProt: Q9H223 Human SwissProt: Q9EQP2 Mouse Unigene: 143703 Human Unigene: 132226 Mouse Unigene: 7379 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
