| 产品编号 | bs-16410R-AF555 |
| 英文名称1 | Rabbit Anti-GTF2A1 alpha/AF555 Conjugated antibody |
| 中文名称 | AF555标记的通用转录因子ⅡA亚基1/TFIIA-α抗体 |
| 别 名 | General transcription factor IIA subunit 1; General transcription factor IIA1; Glucose regulated protein 58kD pseudogene; gtf2a1; TF2A1; TF2AA_HUMAN; TFIIA 42; TFIIA alpha p55; TFIIA alpha p55, isoform 1; TFIIA p35 subunit; TFIIA-42; TFIIAL; Transcription initiation factor IIA alpha chain; Transcription initiation factor IIA subunit 1; Transcription initiation factor TFIIA 42 kDa subunit. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 35/42kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GTF2A1 alpha |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010] Function: TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Subcellular Location: Nucleus. Post-translational modifications: The alpha and beta subunits are postranslationally produced from the precursor form by TASP1. The cleavage promotes proteasomal degradation. Similarity: Belongs to the TFIIA subunit 1 family. Database links: Entrez Gene: 2957 Human Entrez Gene: 83602 Mouse Omim: 600520 Human SwissProt: P52655 Human SwissProt: Q99PM3 Mouse Unigene: 592334 Human Unigene: 275728 Mouse Unigene: 432242 Mouse Unigene: 44436 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
