产品编号 | bs-16380R-AF594 |
英文名称 | Anti-HA1/AF594 |
中文名称 | AF594标记的HA1蛋白抗体 |
别 名 | HLA HA1; HMHA1_HUMAN; HMHA1; KIAA0223; mHag HA-1; Minor histocompatibility antigen HA 1; Minor histocompatibility protein HA 1. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 免疫学 转运蛋白 淋巴细胞 t-淋巴细胞 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | |
产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 125kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human HA1 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: Major histocompatibility complex (MHC) molecules, which include human leukocyte antigens (HLAs), form an integral part of the immune response system. They are cell-surface receptors that bind foreign peptides and present them to cytotoxic T lymphocytes (CTLs). Minor histocompatibility antigens can form an immune response upon recognition by certain T-cells when complexed with MHC molecules. HA-1 (minor histocompatibility protein HA-1), also known as HA-1, HLA-HA1 or HMHA1, is a 1,136 amino acid GTPase activator of Rho-type GTPases. Expressed in dendritic cells, epidermal Langerhans cells, hematopoietic cells, peripheral blood mononuclear cells and all leukemia and lymphoma cell lines, HA-1 is also found in various solid tissues and tumors. Highly phosphorylated, HA-1 contains one Rho-GAP domain, a single phorbol-ester/DAG-type zinc finger and is encoded by a gene located on human chromosome 19p13.3. Function: The minor histocompatibility antigen (mHags), HA-1 is a immunogenic alloantigen shown to be responsible for graft-versus-host disease (GVHD) in HLA-identical bone marrow transplantation. The antigen has two known alleles resulting in a single amino acid polymorphism. The HA-1H allele encodes histidine, whereas the HA-1R allele encodes arginine. Subunit: HA-1 forms a complex with MHC class I HLA-A*0201. Subcellular Location: Cytoplasmic Tissue Specificity: Expressed on cells of the hematopoietic lineage. Detected in dendritic cells and epidermal Langerhans cells. Expressed in peripheral blood mononuclear cells, in all leukemia/lymphoma cell lines. Detected also in some solid tumors and tissues such as cancerous and non-cancerous tissue Similarity: Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Rho-GAP domain. Database links: Entrez Gene: 23526 Human Entrez Gene: 70719 Mouse Omim: 601155 Human SwissProt: Q92619 Human SwissProt: Q3TBD2 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |