| 产品编号 | bs-17063R-HRP |
| 英文名称 | Rabbit Anti-Kindlin/HRP Conjugated antibody |
| 中文名称 | 辣根过氧化物酶标记的整合素相互作用蛋白Kindlin抗体 |
| 别 名 | C20orf42; Chromosome 20 open reading frame 42; DTGCU 2; DTGCU2; FERM1_HUMAN; Fermitin family homolog 1; Fermt1; FLJ20116; FLJ23423; KIND 1; KIND1; Kinderlin; Kindlerin; Kindlin 1; Kindlin syndrome protein; Kindlin-1; Kindlin1; Unc 112 related protein 1; Unc-112-related protein 1; Unc112 related protein; UNC112A; URP 1; URP1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 77kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Kindlin |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: This gene encodes a member of the fermitin family, and contains a FERM domain and a pleckstrin homology domain. The encoded protein is involved in integrin signaling and linkage of the actin cytoskeleton to the extracellular matrix. Mutations in this gene have been linked to Kindler syndrome. [provided by RefSeq, Dec 2009] Function: Involved in cell adhesion. Contributes to integrin activation. When coexpressed with talin, potentiates activation of ITGA2B. Required for normal keratinocyte proliferation. Required for normal polarization of basal keratinocytes in skin, and for normal cell shape. Required for normal adhesion of keratinocytes to fibronectin and laminin, and for normal keratinocyte migration to wound sites. May mediate TGF-beta 1 signaling in tumor progression. Subcellular Location: Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > ruffle membrane. Constituent of focal adhesions. Localized at the basal aspect of skin keratinocytes, close to the cell membrane. Colocalizes with filamentous actin. Upon TGFB1 treatment, it localizes to membrane ruffles. Tissue Specificity: Expressed in brain, skeletal muscle, kidney, colon, adrenal gland, prostate, and placenta. Weakly or not expressed in heart, thymus, spleen, liver, small intestine, bone marrow, lung and peripheral blood leukocytes. Overexpressed in some colon and lung tumors. In skin, it is localized within the epidermis and particularly in basal keratocytes. Not detected in epidermal melanocytes and dermal fibroblasts. DISEASE: Defects in FERMT1 are the cause of Kindler syndrome (KINDS) [MIM:173650]. An autosomal recessive skin disorder characterized by skin blistering, photosensitivity, progressive poikiloderma, and extensive skin atrophy. Additional clinical features include gingival erosions, ocular, esophageal, gastrointestinal and urogenital involvement, and an increased risk of mucocutaneous malignancy. Note=Although most FERMT1 mutations are predicted to lead to premature termination of translation, and to loss of FERMT1 function, significant clinical variability is observed among patients. There is an association of FERMT1 missense and in-frame deletion mutations with milder disease phenotypes, and later onset of complications (PubMed:21936020). Similarity: Belongs to the kindlin family. Contains 1 FERM domain. Contains 1 PH domain. Database links: Entrez Gene: 55612 Human Entrez Gene: 241639 Mouse Omim: 607900 Human SwissProt: Q9BQL6 Human SwissProt: P59113 Mouse Unigene: 472054 Human Unigene: 209784 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Kindlin家族是新近发现的粘着斑蛋白（focal adhesion protein），有3个成员（Kindlin-1、Kindlin-2、Kindlin-3）。Kindlin家族参与整合素活化、细胞迁移、增殖和分化的调控，在临床上与皮肤疾病发生、肿瘤的侵袭、心血管生成、免疫系统功能有密切关系。Kindlins异常可以导致多种遗传性疾病，如Kindlin-1功能异常导致Kindler综合征（Kindler syndrome，KS）和Kindlin-3功能异常导致白细胞黏附缺陷（1eukocyte adhesion deficiency，LAD—HI）。目前已在人类实体肿瘤（乳腺癌、前列腺癌、平滑肌肉瘤）中发现Kindlin-2与肿瘤的侵袭性及耐药性有关。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
