产品编号 | bs-17654R-PE-Cy3 |
英文名称 | Rabbit Anti-SPERT/PE-Cy3 Conjugated antibody |
中文名称 | PE-Cy3标记的精子细胞相关蛋白抗体 |
别 名 | CBY2; Chibby homolog 2; FLJ35810; Novel leucine zipper testicular protein; NURIT; Protein chibby homolog 2; Spermatid associated; Spermatid associated protein; Spermatid flower like structure protein; Testis specific leucine zipper protein nurit. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 细胞生物 发育生物学 转运蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Rat, (predicted: Mouse, Cow, Horse, Rabbit, Sheep, ) |
产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 52kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SPERT |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 |
background: A variety of morphological and molecular changes are required for spermatozoa formation. These steps are temporally guided by the transcription and translation of several testis-specific genes. SPERT (spermatid associated), also known as CBY2 (chibby homolog 2), spermatid flower-like structure protein or NURIT, is a 448 amino acid novel leucine-zipper protein belonging to the chibby family of proteins.Expressed uniquely in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. Containing a leucine-zipper motif and two coiled-coil regions, SPERT is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies. Function: Spermatid-associated protein (SPERT) belongs to the chibby family, SPERT subfamily. It exists as a homodimer and binds to NEK1. There are two named isoforms produced by alternative splicing. Subcellular Location: Cytoplasmic membrane bounded vesicle. Similarity: Belongs to the chibby family. SPERT subfamily. Database links: Entrez Gene: 220082 Human SwissProt: Q8NA61 Human Unigene: 186363 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |