产品编号 | bs-17677R-Gold |
英文名称 | Anti-SPIRE1/Gold |
中文名称 | 胶体金标记的SPIRE1蛋白抗体 |
别 名 | KIAA1135; MGC150621; MGC150622; Protein spire homolog 1; Spir 1; Spir-1; Spir1; SPIR1_HUMAN; SPIRE 1; Spire homolog 1; spire1. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul(10nm 15nm 35nm) |
研究领域 | 细胞生物 信号转导 细胞骨架 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, ) |
产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 85kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 0.4mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SPIRE1 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
产品介绍 |
background: Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]).[supplied by OMIM, Mar 2008] Function: Acts as a actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in vesicle transport processes providing a novel link between actin organization and intracellular transport. Subcellular Location: Cytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Punctate spots in perinuclear region and cytoplasm, co-localised with Rab11. Similarity: Belongs to the spire family. Contains 1 KIND domain. Contains 2 WH2 domains. Database links: Entrez Gene: 56907 Human Entrez Gene: 68166 Mouse Omim: 609216 Human SwissProt: Q08AE8 Human SwissProt: Q52KF3 Mouse Unigene: 515283 Human Unigene: 208723 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |