| 产品编号 | bs-19160R-Gold |
| 英文名称 | Anti-ZFYVE28/Gold |
| 中文名称 | 胶体金标记的锌指蛋白结构域ZFYVE28抗体 |
| 别 名 | hLst2; Lateral signaling target protein 2 homolog; LST2; LST2_HUMAN; LYST2; MGC43699; MGC61592; zfyve28; Zinc finger FYVE domain containing 28; Zinc finger FYVE domain-containing protein 28. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul（10nm 15nm 35nm） |
| 研究领域 | 细胞生物 免疫学 信号转导 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 96kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZFYVE28 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存条件 | Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 产品介绍 |
background: ZFYVE28 is an 887 amino acid protein that contains one FYVE-type zinc finger, which mediates the interaction with PI3P (phosphatidylinositol 3-phosphate). ZFYVE28 functions as a negative regulator of EGFR (epidermal growth factor receptor) by promoting its degradation in endosomes when not monoubiquinated on Lys-87. In the absence of monoubiquination, ZFYVE28 localizes to the early endosome membrane, while it localizes to the cytosol when it is monoubiquinated. There are five isoforms of ZFYVE28 that are produced as a result of alternative splicing events. Function: Negative regulator of epidermal growth factor receptor (EGFR) signaling. Acts by promoting EGFR degradation in endosomes when not monoubiquitinated. Subcellular Location: Cytoplasm > cytosol. Early endosome membrane. Localizes to early endosome membrane in absence of Lys-87 monoubiquitination. Localizes to cytosol when monoubiquitinated. Post-translational modifications: Monoubiquitination at Lys-87 prevents binding to phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes. Similarity: Belongs to the lst-2 family. Contains 1 FYVE-type zinc finger. Database links: Entrez Gene: 57732 Human Entrez Gene: 231125 Mouse Omim: 614176 Human SwissProt: Q9HCC9 Human SwissProt: Q6ZPK7 Mouse Unigene: 292056 Human Unigene: 113388 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
