| 产品编号 | bs-8732R-PE-Cy3 |
| 英文名称 | Rabbit Anti-PIAS1/PE-Cy3 Conjugated antibody |
| 中文名称 | PE-Cy3标记的激活STAT蛋白抑制因子1抗体 |
| 别 名 | AR interacting protein; DDXBP1; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; GBP; Gu binding protein; Gu-binding protein; GU/RH-II; Pias1; PIAS1_HUMAN; Protein inhibitor of activated STAT protein 1; Protein inhibitor of activated STAT, 1; RNA helicase II binding protein; RNA helicase II-binding protein; Zinc finger, MIZ-type containing 3; ZMIZ3. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 转录调节因子 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 81kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PIAS1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
Function: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Subcellular Location: Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton. Tissue Specificity: Expressed in numerous tissues with highest level in testis. Post-translational modifications: Sumoylated. Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene. Similarity: Belongs to the PIAS family. Contains 1 PINIT domain. Contains 1 SAP domain. Contains 1 SP-RING-type zinc finger. Database links: Entrez Gene: 427514 Chicken Entrez Gene: 8554 Human Entrez Gene: 56469 Mouse Omim: 603566 Human SwissProt: O75925 Human SwissProt: O88907 Mouse Unigene: 162458 Human Unigene: 431253 Mouse Unigene: 479179 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
