| 产品编号 | bs-19477R-PE-Cy5 |
| 英文名称 | Anti-NSUN2/PE-Cy5 |
| 中文名称 | PE-Cy5标记的转运RNA（胞嘧啶5）甲基转移酶NSUN2抗体 |
| 别 名 | 5 methycytoisine methyltransferase; D13Wsu123e; FLJ20303; hTrm4; MISU; Myc induced SUN domain containing protein; NOL1/NOP2/Sun domain family 2; NOL1/NOP2/Sun domain family 2 protein; NOL1/NOP2/Sun domain family member 2; NSUN 2; NSUN2; NSUN2_HUMAN; SAKI; Substrate of AIM1/Aurora kinase B; TRM4; tRNA (cytosine 5 ) methyltransferase; tRNA (cytosine 5 ) methyltransferase NSUN2; tRNA (cytosine(34)-C(5))-methyltransferase; tRNA (cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolo; tRNA methyltransferase 4 homolog. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 发育生物学 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 86kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NSUN2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011] Function: RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation. Subcellular Location: Nucleus > nucleolus. Cytoplasm. Concentrated in the nucleolus during interphase and distributed in the perichromosome and cytoplasm during mitosis. Post-translational modifications: Phosphorylated at Ser-139 by Aurora-B/STK12 during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1. DISEASE: A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily. Database links: Entrez Gene: 54888 Human Entrez Gene: 28114 Mouse Omim: 610916 Human SwissProt: Q08J23 Human SwissProt: Q1HFZ0 Mouse Unigene: 481526 Human Unigene: 260009 Mouse Unigene: 27691 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
