| 产品编号 | bs-12599R-PE |
| 英文名称 | Anti-PDK3/PE |
| 中文名称 | PE标记的丙酮酸脱氢酶激酶同功酶3抗体 |
| 别 名 | [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3; CMTX6; GS1-358P8.4; mitochondrial; PDK3; PDK3_HUMAN; Pyruvate dehydrogenase kinase isoenzyme 3; Pyruvate dehydrogenase kinase isoform 3; Pyruvate dehydrogenase kinase isozyme 3; Pyruvate dehydrogenase lipoamide kinase isozyme 3 mitochondrial. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep, ) |
| 产品应用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 47kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDK3 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] Function: Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. Subcellular Location: Mitochondrion matrix. Tissue Specificity: Found almost exclusively in heart and skeletal muscle. Similarity: Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain. Database links: Entrez Gene: 5165 Human Entrez Gene: 236900 Mouse Omim: 602526 Human SwissProt: Q15120 Human SwissProt: Q922H2 Mouse Unigene: 296031 Human Unigene: 12775 Mouse Unigene: 18101 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
