| 产品编号 | bsm-51016M-RBITC |
| 英文名称 | Anti-phospho-FXYD1 (Ser68)/RBITC |
| 中文名称 | 罗丹明（RBITC）标记的磷酸化FXYD1单克隆抗体 |
| 别 名 | FXYD domain containing ion transport regulator 1; Phospholemman; PLM; PLM_MOUSE; PLM_HUMAN; |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 免疫学 神经生物学 通道蛋白 新陈代谢 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 10E2 |
| 交叉反应 | (predicted: Mouse, ) |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 10kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human FXYD1 around the phosphorylation site of Ser68 |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 |
background: PLM (FXYD1)is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. PLM may be phosphorylated by several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Function: May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis. Post-translational modifications: Major plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues. Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK (By similarity). Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA. Similarity: Belongs to the FXYD family. Database links: Entrez Gene: 5348 Human Entrez Gene: 56188 Mouse Omim: 602359 Human SwissProt: O00168 Human SwissProt: Q9Z239 Mouse Unigene: 442498 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
