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Mouse
Anti-Cathepsin D/APC
Cat. Number:
bsm-51237M-APC
Concentration:
1mg/ml Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Background:
Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two-chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer and possibly Alzheimer's disease.
Also known as:
Cathepsin D light chain; CatD; CathepsinD; Cathepsin-D; CLN10; CPSD; CTSD; Lysosomal aspartyl peptidase; MGC2311; CATD_HUMAN.
Specificity:
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Mouse Monoclonal(Clone number:7B4) IgG1, affinity purified by Protein G.
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Reacts with:
(predicted: )
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Immunogen: Recombinant human Cathepsin D.
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Predicted Molecular Weight: 11/38/45kDa.
Storage:
Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.05M PB, pH 7.5. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Application:
Excitation spectrum: 650nm
Emission spectrum: 660nm
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.