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Rabbit  Anti-DBT/AF350
Cat. Number:
bs-14205R-AF350
Quantity size:
100ul
Concentration:
1mg/ml  Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Background:
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Also known as:
BCATE2; BCKAD E2 subunit; Branched chain acyltransferase, E2 component; Dihydrolipoamide branched chain transacylase (E2 component of branched chain keto acid dehydrogenase complex; maple syrup urine disease); Dihydrolipoamide branched chain transacylase; Dihydrolipoamide branched chain transacylase E2; Dihydrolipoyl transacylase; Dihydrolipoyllysine residue (2 methylpropanoyl)transferase; E2; ODB2_HUMAN; E2 component of branched chain alpha keto acid dehydrogenase complex; E2B; Lipoamide acyltransferase component of mitochondrial branched chain alpha keto acid dehydrogenase complex; Mitochondrial branched chain alpha keto acid dehydrogenase transacylase subunit (E2b).
Specificity:
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Rabbit Polyclonal IgG, affinity purified by Protein A.
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Reacts with: (predicted: )
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Immunogen: KLH conjugated synthetic peptide derived from human DBT.
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Predicted Molecular Weight: 46kDa.
Storage:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Application:

Excitation spectrum: 346nm
Emission spectrum: 422nm
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.