G3BP1 Recombinant Mouse mAb (一抗) - WB,Flow-Cyt,ICC/IF | Bioss
货号：bsm-60465M
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概述
产品编号
bsm-60465M
产品类型
重组小鼠单抗
英文名称
G3BP1 Recombinant Mouse mAb
中文名称
Ras GTP酶活化蛋白结合蛋白1重组小鼠单抗
英文别名
G3BP; HDH-VIII; G3BP1_HUMAN; G3BP1; G3BP-1; ATP-dependent DNA helicase VIII (hDH VIII); GAP SH3 domain-binding protein 1; 3.6.4.12; 3.6.4.13; G3BP stress granule assembly factor 1; GTPase activating protein (SH3 domain) binding protein 1; Rasputin homolog; Ras-GTPase-activating protein SH3-domain-binding protein; DNA helicase VIII
抗体来源
Mouse
亚型
IgG1, Kappa
性状
Liquid
纯化方法
affinity purified by Protein G
克隆类型
Recombinant
克隆号
H9B1
检测分子量
68 kDa
浓度
1mg/ml
储存液
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域
保存条件
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq].
产品应用
| 应用 | 已检合格种属 | 预测种属 | 推荐稀释比例 |
|---|---|---|---|
| WB | Human | 1:500-2000 | |
| Flow-Cyt | Human | 1:50-100 | |
| ICC/IF | Human | 1:50-200 |
交叉反应
交叉反应: Human
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靶标
基因名
G3BP1
蛋白名
Ras GTPase-activating protein-binding protein 1
亚基
Binds to the SH3 domain of Ras GTPase-activating protein (RASA1) in proliferating cells. No interaction in quiescent cells Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP (By similarity). Interacts with USP10, and may regulate it. Forms homodimers and oligomers.
亚细胞定位
Cytoplasm. Cytoplasm, cytosol. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
组织特异性
Ubiquitous.
翻译后修饰
Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
相似性
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 RRM (RNA recognition motif) domain.
功能
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
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