APAF1 Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF,ICC/IF | Bioss

bsm-61152R

重组兔单抗、mIHC精品抗体

APAF1 Recombinant Rabbit mAb

凋亡蛋白活性因子-1重组兔单抗

APAF-1; CED4; 6230400I06Rik; Apaf1l; fog; mKIAA0413; APAF_HUMAN; APAF1; KIAA0413; APAF_MOUSE; APAF_RAT; apoptotic peptidase activating factor 1; apoptotic protease activating factor; apoptotic peptidase activating factor

Rabbit

A synthesized peptide derived from human APAF 1: 1-35/1248

IgG

Liquid

affinity purified by Protein A

Recombinant

1D10

142 kDa

140 kDa

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Apaf-1 （Apoptosis protease activating factor-1）调节细胞色素C依赖的Caspase-9原的自动催化活性，导致Caspase-3 的激活和引起凋亡。
Apaf-1在成年人的脾脏、外周血白细胞、肾脏、肺和胎儿脑、肾、肺中高水平表达。

APAF1

Apoptotic protease-activating factor 1

Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain).

Cytoplasm.

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats.

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.