Dnmt3a Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF,Flow-Cyt,ICC/IF | Bioss

bsm-61213R

重组兔单抗、mIHC精品抗体

Dnmt3a Recombinant Rabbit mAb

DNA甲基转移酶-3α重组兔单抗

DNM3A_HUMAN; DNMT3A; Cysteine methyltransferase DNMT3A; DNA methyltransferase HsaIIIA (DNA MTase HsaIIIA | M.HsaIIIA); 2.1.1.37; DNA methyltransferase 3 alpha; DNA (cytosine-5-)-methyltransferase 3 alpha

Rabbit

A synthesized peptide derived from human DNMT3A: 609-624/912

IgG

Liquid

affinity purified by Protein A

Recombinant

4F12

102 kDa

130 kDa

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

肿瘤组织存在DNA甲基化紊乱，包括与细胞增殖周期密切相关的癌基因低甲基化和抑癌基因高甲基化DNA甲基转移酶(Dnmt)参与甲基化的形成(主要是Dnmt3a和Dnmt3b)和维持（主要是Dnmt1）
(isoform CRA_b)(DNA methyltransferase MmuIIIA)
(DNA MTase MmuIIIA)
(M.MmuIIIA)

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development.DNA methylation is coordinated with methylation of histones.

DNMT3A

DNA(cytosine-5)-methyltransferase 3A

Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with UBC9, PIAS1 and PIAS2. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain. Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Interacts with MPHOSPH8. Interacts with histone H3 that is not methylated at 'Lys-4' (H3K4).

Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin.

Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.

Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.