E2F1 Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF,IP,Flow-Cyt | Bioss

bsm-61369R

重组兔单抗

E2F1 Recombinant Rabbit mAb

转录因子E2F-1重组兔单抗

E2F-1; RBAP1; RBBP3; RBP3; Tg(Wnt1-cre)2Sor; mKIAA4009; E2F1_HUMAN; E2F1; PBR3; Retinoblastoma-associated protein 1 (RBAP-1); Retinoblastoma-binding protein 3 (RBBP-3); pRB-binding protein E2F-1; E2F1_MOUSE; E2F1_RAT; E2F transcription factor 1; retinoblastoma-binding protein 3 (E2F-like)

Rabbit

A synthesized peptide derived from human E2F1: 365-400

IgG

Liquid

affinity purified by Protein A

Recombinant

4D13

47 kDa

60 kDa

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

E2F1—属于调节性转录因子E2F家族。有学者认为：E2F-1既可作为癌基因起作用，又可作为抑癌基因起作用。其不同可能由细胞中其他生长促进或抑制性蛋白质水平和（或）活性决定，同时与细胞所处环境及器官特异性有关。在控制细胞周期和肿瘤抑制基因蛋白的活性方面起关键作用。

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.

E2F1

Transcription factor E2F1

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A. Interacts with TRIM28; the interaction inhibits E2F1 acetylation through recruiting HDAC1 and represses its transcriptional activity. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity. Interacts with BIRC2/c-IAP1 (via BIR domains). Interacts with human cytomegalovirus/HHV-5 protein UL123.

Nucleus.

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.

Belongs to the E2F/DP family.

Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.