Oct4 Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF,Flow-Cyt | Bioss
Rrmab?兔单抗
货号：bsm-63076R
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概述
产品编号
bsm-63076R
产品类型
重组兔单抗
英文名称
Oct4 Recombinant Rabbit mAb
中文名称
胚胎干细胞关键蛋白重组兔单抗
英文别名
OCT3; OCT4; OCT4Borf1; OTF-3; OTF3; OTF4; Oct-3; Oct-4; Oct3/4; NF-A3; Oct-3/4; Otf-4; Otf3-rs7; Otf3g; PO5F1_HUMAN; POU5F1; Octamer-binding protein 3 (Oct-3); Octamer-binding protein 4 (Oct-4); Octamer-binding transcription factor 3 (OTF-3); PO5F1_MOUSE; POU class 5 homeobox 1; POU domain class 5, transcription factor 1
抗体来源
Rabbit
免疫原
A synthesized peptide derived from human Oct 4: 20-48
亚型
IgG
性状
Liquid
纯化方法
affinity purified by Protein A
克隆类型
Recombinant
克隆号
6B9
理论分子量
39 kDa
检测分子量
42 kDa
浓度
1mg/ml
储存液
10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
研究领域
SWISS
Gene ID
保存条件
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
数据库链接
产品介绍
OCT4B-190蛋白能够增强细胞在应激条件下抵抗凋亡的能力。OCT4基因在干细胞的增殖、分化、应激反应、凋亡过程等多个生物学过程中发挥着重要的作用。
背景资料
Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
基因名
POU5F1
蛋白名
POU domain, class 5, transcription factor 1
亚基
Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2.
亚细胞定位
Nucleus.Note=Expressed in a diffuse and slightly punctuate pattern.
组织特异性
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
翻译后修饰
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation (By similarity).
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation (By similarity).
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
相似性
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
功能
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
产品应用
| 应用 | 已检合格种属 | 预测种属 | 推荐稀释比例 |
|---|---|---|---|
| WB | Human | Mouse | 1:500-2000 |
| IHC-P | Human, Mouse | 1:100-500 | |
| IHC-F | Human, Mouse | 1:100-500 | |
| IF | Human, Mouse | 1:100-500 | |
| Flow-Cyt | Human | Mouse | 1:50-100 |
交叉反应
交叉反应: Human (predicted: Mouse)
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