Name: 转录因子MYB相关蛋白B即用型免疫组化试剂盒（MYBL2 Ready-To-Use IHC Kit）
Brand: 北京博奥森生物科技有限公司
SKU: IHC0105
Availability: InStock

概述

产品编号

IHC0105

英文名称

MYBL2 Ready-To-Use IHC Kit

中文名称

转录因子MYB相关蛋白B即用型免疫组化试剂盒

英文别名

B-Myb; BMyB; MYB L2; Myb related protein B; Myb-like protein 2; Myb-related protein B; MybB; MYBB_HUMAN; MYBL 2; Mybl2; v myb avian myeloblastosis viral oncogene homolog like 2; v myb myeloblastosis viral oncogene homolog (avian) like 2; v myb myeloblastosis viral oncogene homolog like 2.

研究领域

Cancer > Oncoproteins/suppressors > Oncoproteins > Transcription factors

Cell Biology > Cell Cycle > Cell differentiation

Epigenetics and Nuclear Signaling > Cell cycle > Cell Differentiation

Epigenetics and Nuclear Signaling > Transcription > Other factors

Epigenetics and Nuclear Signaling > Transcription > Transcription Factors

描述

IHC0105 is a ready-to-use IHC kit for staining of MYBL2. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

适用样品基质

FFPE tissue

应用范围

Immunohistochemistry

保存条件

Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

注意事项

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

数据库链接

Entrez Gene : 4605 Human

Entrez Gene : 17865 Mouse

Entrez Gene : 296344 Rat

Omim : 601415 Human

SwissProt : P10244 Human

SwissProt : P48972 Mouse

Unigene : 179718 Human

Unigene : 4594 Mouse

Unigene : 20824 Rat

产品介绍

产品组分及规格

编号	组分	规格	浓度	储存
1	PBS缓冲液（干粉）	2 L×2	20x	室温
2	抗原修复缓冲液	20 ml	100x	2-8℃
3	内源性过氧化物酶阻断剂	3 ml	RTU	2-8℃
4	封闭工作液	3 ml	RTU	2-8℃
5	一抗（Rat MYBL2 Rabbit pAb）	6 ml	RTU	2-8℃
6	二抗（HRP-Goat anti-Rabbit IgG pAb）	6 ml	RTU	2-8℃
7	DAB kit（20×）显色液	0.3 ml	RTU	-20℃
8	DAB kit（20×）稀释液	0.3 ml	RTU	-20℃
9	复染试剂	5 ml	RTU	室温
10	封片剂	5 ml	RTU	室温
11	对照切片（大鼠脑）	1张	RTU	室温
12	说明书	1份

背景

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

石蜡包埋组织的免疫组织化学方案

1. 脱蜡水化

石蜡切片置于新鲜二甲苯中浸泡脱蜡3次，每次15 min；依次置于不同浓度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸馏水洗涤5 min，重复3次。

2. 抗原修复

沸水浴修复：将100×抗原修复缓冲液（试剂2）用蒸馏水稀释成1×抗原修复缓冲液，放入修复盒中并提前加热至95-100℃（注意盖好以防液体蒸发），然后将切片放入修复盒中，在沸水浴环境中保持外沸状态15 min，室温自然冷却；用PBS缓冲液（试剂1，将干粉溶解在2L蒸馏水中）清洗5 min，重复3次。

3. 阻断内源性过氧化物酶

用吸水纸吸去玻片上多余的液体，用免疫组化笔在组织周围画圈，加入2-4滴内源性过氧化物酶阻断剂（试剂3），室温下置于湿盒中孵育15 min，用PBS洗涤5 min，重复3次。

4. 血清封闭

用吸水纸吸去玻片上多余的液体，加入2-4滴封闭工作液（试剂4），置于湿盒内37℃封闭20 min，以减少非特异性染色。

5. 一抗孵育

用吸水纸吸去玻片上多余的液体，加入2-4滴大鼠MYBL2兔多抗工作液（试剂5），置于湿盒中，4℃孵育过夜或37℃孵育1-2 h。

6. 复温

4℃孵育过夜后，室温下复温15 min (若在室温下孵育一抗，则直接进入下一步清洗)；用PBS洗涤5 min，重复3次。

7. 二抗孵育

用吸水纸吸玻片上多余的液体，加入2-4滴HRP标记羊抗兔IgG工作液（试剂6），置于湿盒中，37℃孵育1-2 h；用PBS洗涤5 min，重复3次。

8. 显色

用吸水纸吸去玻片上多余的液体，在每张切片上滴加约50 μL新配制的DAB工作液（试剂7:试剂8:PBS=1:1:18），作用3-5 min。显微镜下观察结果，达到合适的显色强度后，用蒸馏水冲洗切片以终止反应，用蒸馏水冲洗5 min，重复3次。

9. 复染

滴加适量复染试剂（试剂9）复染3-5 min，蒸馏水冲洗5 min，滴加盐酸酒精分化约30 s，蒸馏水洗涤5 min，重复2次。

10. 脱水封片

将玻片依次置于不同浓度（70%、80%、90%、95%、100%）乙醇，各5 min；然后置于新鲜二甲苯中浸泡脱蜡3次，每次15 min。用吸水纸吸去多余的二甲苯，滴加适量封片剂（试剂10）在组织上，将盖玻片盖在组织上，避免产生气泡。

注意事项

1. 建议检测时进行阴性及阳性对照，以提高实验的可靠性。

2. 本品中的配套试剂，请不要用其他生产商产品替换使用。

3. DAB为致癌物质，请采取必要的防范措施。

4. PBS洗涤液（试剂1）配制后在4℃可保存一周；抗原修复液（试剂2）及显色剂（试剂7和8）的工作液需每次实验时现用现配。

*5. 发表论文时引用本产品的写作建议 "IHC0105R, Bioss Antibodies"。引用示例: “Rat tissue sections using Rat MYBL2 IHC Kit (IHC0105R, Bioss Antibodies) were stained for MYBL2 according to the manufacturer's instructions.”

背景资料

Myb-Related Protein B (MYBL2), a member of the MYB family of transcription factor genes, is a nuclear protein involved in the regulation of cell survival, proliferation, and differentiation. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes.

基因名

MYBL2

蛋白名

Myb-related protein B

亚基

Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL22.

亚细胞定位

Nucleus.

组织特异性

Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.

翻译后修饰

Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.

相似性

Contains 3 HTH myb-type DNA-binding domains.

功能

Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.