MCM2 Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF,Flow-Cyt,ICC/IF | Bioss
Rrmab?兔单抗
货号：bsm-63311R
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概述
产品编号
bsm-63311R
产品类型
重组兔单抗、mIHC精品抗体
英文名称
MCM2 Recombinant Rabbit mAb
中文名称
微小染色体维持缺陷蛋白2重组兔单抗
英文别名
BM28; CCNL1; CDCL1; D3S3194; DFNA70; MITOTIN; cdc19; Mcmd2; mKIAA0030; MCM2_HUMAN; MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28; 3.6.4.12; KIAA0030; MCM2_MOUSE; minichromosome maintenance complex component 2; minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin); MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
抗体来源
Rabbit
免疫原
A synthesized peptide derived from human MCM2: 41-65
亚型
IgG
性状
Liquid
纯化方法
affinity purified by Protein A
克隆类型
Recombinant
克隆号
7F5
理论分子量
102 kDa
检测分子量
130 kDa
储存液
10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
SWISS
Gene ID
保存条件
Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
数据库链接
产品介绍
微小染色体维持缺陷蛋白2又称微型染色体维持蛋白2或丝裂蛋白，MCM-2是细胞周期阶段的特异性标志物,在进入细胞周期的细胞中均有表达，在细胞的增值、分化中起到一定的作用
背景资料
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.
基因名
MCM2
蛋白名
DNA replication licensing factor MCM2
亚基
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with KAT7 and DBF4. May interact with MCM10.
亚细胞定位
Nucleus (Probable).
翻译后修饰
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
相似性
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
产品应用
| 应用 | 已检合格种属 | 预测种属 | 推荐稀释比例 |
|---|---|---|---|
| WB | Human, Mouse, Rat | 1:1000-2000 | |
| IHC-P | Human, Mouse, Rat | 1:200-1000 | |
| IHC-F | Human, Mouse, Rat | 1:200-1000 | |
| IF | Human, Mouse, Rat | 1:200-1000 | |
| Flow-Cyt | Human | Mouse, Rat | 1:100-200 |
| ICC/IF | Human | Mouse, Rat | 1:100-500 |
交叉反应
交叉反应: Human, Mouse, Rat
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